Part:BBa_K1398005
NemR Upstream Intergenic Region
This sequence is found upstream of several NemR regulated genes. It was created for use as a TNT-detection mechanism. It contains the bases from 220+ to the start codon, which include a promoter and RBS. This region should allow regulation of gene preoducts through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.
An iLOV reporter was added to this construct to characterize the response to concentrations of TNT. This part can be found here:
https://parts.igem.org/Part:BBa_K1398004
The full article detailing the characterisation method can be found here:
http://2014.igem.org/Team:Exeter/Detection
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |